Fig. 3

Tau-immunopositive inclusions. Anti-AT8 immunohistochemistry reveals prominent neuronal and astrocytic aggregations in the deep layer of the cerebral cortex (a). Tau aggregation is also severe in the medullary reticular formation (b) and the anterior horn of the spinal cord (c). The intramedullary tract of the hypoglossal nerve shows intraaxonal tau aggregation (d). The tau aggregations were four-repeat tau-immunopositive (a). The neuronal inclusions comprised globose-type neurofibrillary tangles (f) and pretangles (g), and a subset of the motor neurons in the spinal anterior horn show tau inclusions with fine, fibrous configurations (h). The astrocytes demonstrate miscellaneous aggregations, including bush-like inclusions (i), and depositions within the distal (j) and proximal foot processes (k). Oligodendroglial inclusions are abundant in the cerebral white matter (l). Astrocytic inclusions are fundamentally argyrophilic (m). The hippocampal pyramidal neurons show argyrophilic grains (n). a–d, f–l Anti-AT8 immunohistochemistry, e anti-RD4 immunohistochemistry, and m, n Gallyas–Braak staining were used. Scale bars: a–d 100 μm, e, j, k 20 μm f–i, l–n 10 μm