Fig. 4

RRM2 deletion recues mutant MATR3 toxicity in vivo. a Quantification of egg-to-adult viability in flies expressing double mutants: F115C ΔRRM2 and S85C ΔRRM2. Constitutive ubiquitous expression of ΔRRM2 in the mutant background completely rescued mutant MATR3-mediated developmental toxicity (n = 3, One-way ANOVA). b Quantification of egg-to-adult viability in flies expressing double mutants: F115C ΔRRM1 and S85C ΔRRM1. Constitutive ubiquitous expression of ΔRRM1 in the mutant background is not sufficient to rescue mutant MATR3-mediated developmental toxicity (n = 3, One-way ANOVA). c Kaplan–Meier survival curve of adults ubiquitously expressing MATR3 deletion mutants in F115C background and d S85C background under conditional driver (TubGS-Gal4). ΔRRM1 and ΔRRM2 significantly increased longevity of adults expressing the MATR3 mutants (n = 50, Log-rank Mantel-Cox test). e Immunoblot of NP40-soluble and NP40-insoluble fractions of MATR3 from lysates of flies expressing full-length WT and mutant MATR3, and corresponding deletion mutants. α-tubulin is used as loading control. f Quantification of the insoluble/soluble fractions of full-length MATR3 and deletion mutants, ΔRRM1 and ΔRRM2, in the background of WT, g F115C and h S85C. Deletion of RRM2 significantly increased the solubility of MATR3 WT, F115C and S85C (n = 4 per group; One-way ANOVA) Error bars indicate S.E.M. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001