Skip to main content
Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Acute brain injuries trigger microglia as an additional source of the proteoglycan NG2

Fig. 5

NG2 gene-activated microglia with NG2 protein expression within short time after SWI. a Overview of transgene structures of NG2tdTxCXCREGFP mice used in SWI model. b Experimental design: After SWI, NG2tdTxCXCREGFP mice were injected with TAM from 0 to 2 dpi, and analyzed at 3, 7, or 28 dpi. c–f Confocal images showing NG2 immunoreactivity in tdT+, EGFP+ cells at the lesion site at 3 dpi (c, d), and 7 dpi (e, f). EGFP (triangles) and tdT (open arrowheads) were mostly expressed in different cell types, with exceptions of a few tdT+ EGFP+ cells detected at the lesion site (arrowheads). The immunoreactivity to NG2 was detected in some tdT+ EGFP+ cells (arrows indicate the NG2 positive processes) at 3 dpi (d, magnifying the white box in c) but undetectable at 7 dpi (f, magnifying the white box in e), whereas most cells expressing only tdT (arrowheads) were always NG2 immunopositive with some exceptions indicating their differentiation into mature oligodendrocytes. g Confocal images showing a tdT+ EGFP+ cell found at 28 dpi, with the orthogonal view (right image). Scale bars = 20 μm in c, e, f, and 10 μm in d, g

Back to article page