Fig. 3

NG2 protein expression in some microglia after tMCAO. a Overview of transgene structures of the triple transgenic mouse line NG2^tdTxCXCR^EGFP used in tMCAO model. b Experimental plan: NG2^tdTxCXCR^EGFP mice were injected with tamoxifen (TAM) for three consecutive days from 2 to 4 days post injury (dpi) and were analyzed at 9 dpi in the tMCAO model. c Micrographs showing EGFP⁺ cells and tdT⁺ cells never overlapped in the contralateral hemisphere. TdT⁺ cells displayed either morphology of oligodendrocyte lineage cells (open arrowheads) or vascular pericytes (open triangle). d and e Confocal images in the infarct region with tdT⁺ EGFP⁺ cells immuno-positive (d) or immuno-negative (e) for NG2. Scale bars = 50 μm in c, 20 μm in d and e