Fig. 2

No differentiation of NG2-expressing cells into microglia after tMCAO. a Scheme of transgene structures of the triple transgenic mouse line NG2^tdTxCXCR^EGFP used in tMCAO model. b Experimental plan: NG2^tdTxCXCR^EGFP mice were occluded (tMCAO) 10 days after the 3-day injection of tamoxifen (TAM) and were analyzed at 7 days post injury (dpi). c- d Representative confocal images from injured striatum showed that virtually all tdT⁺ cells (including cells in oligodendrocyte lineage and pericytes labeled by PDGFRβ) were not co-expressing EGFP, and intermingled cells could be distinguished by the Z-stack images (d, orthogonal view of the corresponding white box in c). Very rarely tdT⁺ EGFP⁺ cells (2 cells found in 18 brain slices from three mice, 6 slices per mouse) were detected as indicated in e (orthogonal view of the corresponding white box in c). Scale bar = 20 μm