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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Tau reduction in aged mice does not impact Microangiopathy

Fig. 2

Tau protein in vasculature from transgenic mice. a Tissue sections from rTg4510 or wild-type controls at 15 months of age were labeled for tau (HT7) and endothelial cells (Glut1). b An enlarged view of the box from (a) shows the extent of tau pathology at this age in transgenic (TG) and not wild-type (WT) mice. c Numerous blood vessels were observed to be closely associated with tau. d Western blotting of total cortex and isolated vessels indicates tau is present in vessels, but not other neuronal components such as NeuN. Glut1 is highly enriched. Vessels and cortex protein were run on the same blot, but separated here for clarity. e Tau bioactivity in a biosensor cell assay shows increased tau bioactivity in transgenic brain versus wild-type (Repeated Measures ANOVA, Sidak’s post hoc, p = 0.003) and in transgenic blood vessels versus wild-type (Sidak’s post hoc, p = 0.01). f Tau protein ELISA measures in total brain versus blood vessel protein. g Expression of the human Mapt transgene was observed by in situ hybridization in neurons but did not co-localize with Glut1-positive endothelial cells. Image is of a single plane captured using a confocal microscope. All graphs are plotted with means +/− standard deviations. * indicates p < 0.05, ** p < 0.01

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