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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Tau reduction in aged mice does not impact Microangiopathy

Fig. 1

Tau protein in human vasculature. a Blood vessel isolations containing capillaries were validated by immunofluorescent labeling. DAPI-positive nuclei were also positive for endothelial cell markers such as von Willebrand factor (vWF) and zona occludins (ZO-1). b Isolates also contained basement membrane (collagen IV, ColIV) and some vessels were arteriolar in origin, indicated by the presence of smooth muscle actin (SMA). c Glut1 labeled endothelial cells were also occasionally observed to be surrounded by tau (HT7 antibody). d Western blotting of blood vessel isolations from human temporal cortex samples with varying degrees of tau pathology (Braak stage) indicates that vessels are frequently positive for tau (DAKO), but not other neuronal components (NeuN). Vessels are also enriched in Glut1 compared to total brain extracts. e When applied to a tau biosensor cell assay, vessels appear to retain their bioactivity with AD vessels exhibiting elevated seeding potential compared to controls (Mann-Whitney U test, p = 0.007, A.U. = arbitrary units). f Total frontal cortex protein from subjects with frontotemporal lobar dementia (FTLD) with tau exhibited greater bioactivity than control brains (Student’s t test, p = 0.038). g Total tau protein in total brain versus blood vessel protein preparations as measured by ELISA. All graphs are plotted with means +/− standard deviations. * indicates p < 0.05, ** p < 0.01

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