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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease

Fig. 5

Overexpression of STI1 in 5xFAD mice does not affect APP processing, however levels of Aβ regulating enzymes are altered. a Representative Western blot for membrane bound full length APP and C-terminal APP fragments in cortices of 3–5-month old male 5xFAD and TgA-5xFAD mice. b Densitometric quantification of C-terminal fragment protein levels relative to full length APP, presented as percentage of total full-length APP [Signal intensity of the α or β fragment/signal intensity of full-length APP] × 100). At least 3 mice/genotype were used for experiments. Data were analyzed with Two-way ANOVA with Sidak’s post-hoc comparisons. On each graph, black circles represent individual 5xFAD mice and black squares are individual TgA-5xFAD mice. c Protein levels of BACE1 in cortical tissue from 3 to 5-month old 5xFAD and TgA-5xFAD mice and d, densitometric analysis. e Representative immunoblot and quantification f, for Aβ-degrading enzyme, matrix-metalloproteinase-2 (MMP-2). g Representative immunoblot and h quantification, for Aβ-degrading enzyme neprilysin. i Representative immunoblot and j quantification, for Aβ-degrading enzyme, Insulin-degrading enzyme (IDE). For C-J, N = 5 mice/genotype. Data are presented as mean ± SEM. Experiments were analyzed using unpaired t-test. *p < 0.05. Raw data available at: https://doi.org/10.6084/m9.figshare.12115758

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