Fig. 5
From: P53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer’s disease
p53 oligomers colocalize with p53, but not tau. Tau oligomers colocalize with p53 and P-p53. a Representative confocal image of untreated C57BL/6 primary neurons probed with anti-p53 (green), and I11 (oligomer specific). Magnified ROI shows p53 signal in the nucleus and no oligomers. b Representative immunofluorescent confocal images of C57BL/6 primary neurons treated with AFL-p53O were probed with anti-p53 (green). Peri-nuclear colocalization between AFL-p53O and endogenous WT p53 is observed at 1 h and 4 h. Magnified ROI with colocalized pixel maps show colocalization between AFL-p53O and endogenous p53. c Representative confocal image of untreated C57BL/6 primary neurons probed with anti-p53 (red) and anti-tau (green). Magnified ROI shows p53 signal in the nucleus and on the microtubules. d Confocal Imaging of C57BL/6 primary neurons treated with AFL-p53O were immunofluorescent probed with anti-tau (red) and show no colocalization between tau and AFL-p53O. At 4 h, magnified ROI show peri-nuclear p53 signal that also appears to travel on the microtubules. e Representative confocal image of untreated C57BL/6 primary neurons probed with anti-p53 (green) and anti-tau (red). Magnified ROI shows p53 signal in the nucleus and on the microtubules. f Confocal Imaging of C57BL/6 primary neurons treated with AFL-tauO were immunofluorescently probed with anti-p53 (green) and show colocalization between AFL-tauO and endogenous p53. Magnified ROI show peri-nuclear colocalization at both concentrations and timepoints. g Representative confocal image of untreated C57BL/6 primary neurons probed with anti-P-p53 (green), and anti-tau (red). Magnified ROI shows P-p53 signal in the nucleus and on the microtubules. h Confocal Imaging of C57BL/6 primary neurons treated with AFL-tauO were immunofluorescently probed with anti-P-p53 (green) and show colocalization between AFL-tauO and endogenous P-p53. Magnified ROI show peri-nuclear colocalization at both concentrations at 1 h. i Fluorescent intensity of P-p53 shows a significant increase at 1 μM tauO concentration as compared to untreated. Keyence microscope, 60X magnification, scale bar = 50 μm