Fig. 4
From: P53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer’s disease
p53 oligomers detected in aged tau mouse models, but not in aged control mice. a Representative immunofluorescent confocal images of cortex of 14-month-old htau, 5-month-old P301L, and 9-month-old C57BL/6 mice. Brain tissue probed with anti-p53 (red) and anti-I11 (green; oligomer-specific) in merge panel. b Magnified ROI from merged panel in A. (htau ROI 1 and 2) Peri-nuclear colocalization between p53 and I11 is shown with other oligomers in the vicinity. (P301L ROI 1 and 2) Peri-nuclear, circular colocalization between p53 and I11 is shown with other oligomers in the vicinity. (C57BL/6 ROI 1) No colocalization between p53 and I11 is detected in C57BL/6 mice. Keyence Microscope 60X magnification. Scale bar = 50 μm. c Graph depicting immunofluorescent intensity for I11 oligomer (n = 2 in technical triplicate) and (d) p53 intensities from htau and C57BL/6 mice (n = 2 in technical triplicate). Keyence Microscope 60X magnification. Scale bar = 50 μm. e Western blot of aged C57BL/6, htau, P301L, and Tau KO mice probed with anti-p53. f Densitometry of p53 bands shows a significant increase in p53 in htau mice at multiple bands compared to C57BL/6 and Tau KO mice (n = 3). g Western blot of same mice probed with anti-MDM2 (90 kD). h Densitometry of MDM2 from G shows significant increase in MDM2 in htau compared to all other mouse models. P301L also show significantly more MDM2 compared to control and Tau KO mice. (n = 3 in technical duplicate)