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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Lipid-specific IgMs induce antiviral responses in the CNS: implications for progressive multifocal leukoencephalopathy in multiple sclerosis

Fig. 5

Lipid-specific IgM mediated antiviral responses are not sulfatide-dependent and identification of similar antibodies in human repertoire. a, b O4 staining in WT and Cst−/− mouse cultures respectively. c Ifnb1 expression in DIV24 Cst+/+, Cst+/− and Cst−/− cultures treated 6 h with A4CD or O4. Data presented as mean fold change compared to untreated control ± SEM and analysed by two-way ANOVA. d-g Representative images of binding patterns of A4CD, O4, O1 and A2B5 in DIV24 rat cultures. h, i ISG expression in DIV24 rat cultures treated 24 h with A4CD, O4, O1 and A2B5. Data presented as mean fold change compared to untreated control ± SEM and analysed by one-way ANOVA with Tukey’s post hoc test. Significant difference compared to A4CD denoted as *p < 0.05, n = 3 for all conditions. j-l Screening of patient-derived human IgM antibodies (hIgM’s) j Ifnb1 expression 2 h post-treatment. k Cxcl10 expression 24 h post-treatment. Data represent two technical replicates from one biological n (DIV24 mouse cultures) and are presented as mean ± SD. l Immunocytochemical analysis of DIV24 mouse cultures pre-treated 24 h with hIgM’s and infected with SFV. Data presented as mean ± SD and represent a biological n of 2 for all conditions except sample 3 (n = 1)

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