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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Single-cell mass cytometry reveals complex myeloid cell composition in active lesions of progressive multiple sclerosis

Fig. 4

Confirmation of differentially phenotypic diversity of myeloid cells in active lesions. a, b The overlaid t-SNE plot of ten NAWM and eight active lesion samples (Exp-II) (a), or of eight NAWM and nine active lesion samples (Exp-III) (b). The 2D t-SNE maps were generated based on expression levels of TYPE markers of Exp-II (Supplementary Table 8, a) or of Exp-III (Supplementary Table 8, b). The coloring indicates 12 defined clusters representing diverse myeloid cell phenotypes. Heat map cluster demonstrates the expression levels of TYPE markers used for t-SNE embedding. c, d Box-plots show frequencies (%) of all defined clusters in Exp-II (c) and Exp-III (d). Differentially abundant clusters (C1, C5, and C6, Exp-II; C1, C3 and C9, Exp-III) between active lesions and NAWM were found. A FDR-adjusted P value < 0.05 was considered statistically significant, determined using GLMM. Boxes extend from the 25th to 75th percentiles. Whisker plots show the min (smallest) and max (largest) values. The line in the box denotes the median. Each dot represents the value of each sample. e, f Reduced-dimensional single-cell t-SNE maps highlight differentially abundant clusters of Exp-II (e) and Exp-III (f). g, h Snail plot shows marker expression levels of each differentially abundant clusters (of all samples), in comparison to hoMG cluster C6 of Exp-II (g) or C9 of Exp-III (h). Significantly differential expressed markers are in bold. Two-tailed, unpaired t-test followed a correction for multiple comparisons using the Holm-Šídák method. Adjusted p-value < 0.05 is considered statistically significant

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