Fig. 4

Upregulation of ß-hexosaminidase activity prevents nigrostriatal accumulation of AAV6-overexpressed alpha-synuclein. a Triton X-soluble (TRT) cytosolic fractions, and Triton X-insoluble/SDS-soluble membrane enriched fractions (SDS) were prepared by sequential ultracentrifugation of homogenized spot-dissected SN lysates. b, d Representative immunoblots of TRT-soluble aSYN in CTRL+aSYN and HEX+aSYN AAV6-injected SN (b) and striatum (d) relative to their non-injected control hemispheres. c, e Densitometric quantifications of aSYN normalized by GAPDH, expressed as fold change from the non-injected hemisphere per animal (dotted line) in the SN (c) and striatum (e) (*:p < 0.05, two-tailed t-test). f, h Representative immunoblots of SDS-soluble aSYN in CTRL+aSYN and HEX+aSYN AAV6-injected SN (f) and striatum (h) relative to their non-injected control hemispheres. g Densitometric quantifications of SN SDS-soluble aSYN normalized by GAPDH, compared to the non-injected control hemisphere per animal (*:p < 0.05, paired ratio matched sample t-test). i Densitometric quantifications of STR SDS-soluble aSYN normalized by GAPDH, expressed as fold of non-injected control hemisphere. n = 5–7 / group for all graphs