Fig. 7

Microglia activation state does not change upon scFvMC1 treatment. For analysis of activation state, sections were immunolabeled for CD68 and Iba-1 and imaged. a Representative image of microglia from P301S controls (n = 6) (upper images) and AAV-scFv-MC1 treated mice (n = 6) (lower images), in the stratum radiatum: CD68 lysosomal protein (red puncta), Iba1 (green), nuclei stained with DAPI (blue). b Quantification of Iba-1 and CD68 showed no significant differences between controls and treated mice (p = 0.0931 and p = 0.7771 respectively, non parametric Mann-Whitney test). c Representative image of microglia from JNPL3 controls (n = 13) (upper images) and AAV-scFv-MC1 treated mice (n = 13) (lower images), in the stratum radiatum. d Quantification of Iba-1 and CD68 showed no significant differences between controls and treated mice in JNPL3 mice (p = 0.8857 and p = 0.4857 respectively, non parametric Mann-Whitney test). All images were obtained using Zeiss 880 confocal laser microscope, scale bar: 20 μm. Graphs are expressed as integrated density, and means +/− SEM