Fig. 6

Phospho-Threonine-231 and MC1-tau immunoreactivity in brain. Brains from P301S and JNPL3 animals were harvested and stained with either anti tau phospho-Thr231 antibody (A) or anti-MC1-tau (B). (A): a, b, d, e Representative images of CA1 hippocampal cell layer and entorhinal cortex (EC) stained with anti-pThr231. Control mice received AAV1-CAG-eGFP injection (n = 13). Treated mice were injected with AAV1-CAG-scFvMC1 (n = 13). (A): c,f Semi-quantification of percentage of area stained by RZ3 shows a trend of reduction in the AAV1-CAG-scFvMC1 injected group, in the CA1 region of hippocampus in the JNPL3 (c, p = 0.1128, unpaired t test with Welch’s correction); no trend to reduction is detected in the entorhinal cortex (EC) (f, p = 0.3458, unpaired t test with Welch’s correction). (A): g, h, j, k Representative images of the CA1 region and entorhinal cortex (EC) from P301S; pThr-231 staining was performed as above. Control mice received AAV1-CAG-eGFP injection (n = 6), treated mice were injected with AAV1-CAG-scFvMC1 (n = 6); (A) i, l Semi-quantification of percentage of area stained by RZ3 in P301S mice (i, p = 0.2403; l, p = 0.2251; non parametric Mann-Whitney test). (B): a, b, d, e Representative images of CA1 hippocampal cell layer and entorhinal cortex (EC) stained with anti-MC1 antibody. Control mice received AAV1-CAG-eGFP injection (n = 13). Treated mice were injected with AAV1-CAG-scFvMC1 (n = 13) (c, f) Semi-quantification of percentage of area stained by MC1 shows a trend of reduction in the AAV1-CAG-scFvMC1 injected group, in the CA1 region of hippocampus (c, p = 0.0996; unpaired t test with Welch’s correction); no reduction is detected in the entorhinal cortex (EC) (f, p = 0.9558; unpaired t test with Welch’s correction) in JNPL3. (g, h, j, k) Representative images of the CA1 region and the entorhinal cortex (EC) from P301S; MC1 staining was performed as above. Control mice received AAV1-CAG-eGFP injection (n = 6), treated mice were injected with AAV1-CAG-scFvMC1 (n = 6). (i, l) Semi-quantification of percentage of area stained by MC1 in P301S mice (i, p = 0.4127; l, p = 0.8413; non parametric Mann-Whitney). (Olympus BH-2 bright field microscope; scale bar: 100 μm). Graphs are expressed as % Control area stained, and means +/− SEM