Fig. 4

Soluble and oligomeric tau levels in P301S mice are significantly reduced. a, b, c, d P301S mice were injected at 2 month of age and sacrificed 4 months post-injection. Levels of total and phosphorylated soluble tau (pThr231, pSer202, pSer396/404) were tested on heat stable preparations (hsp) from 3 different brain regions, using low-tau ELISA: (a) cortex (ctx), (b) hindbrain (HB) and (c) hippocampus. In all three regions analyzed, soluble tau levels showed a significant reduction in the AAV1-CAG-scFvMC1 group (scFvMC1) (n = 6) compared to the AAV1-CAG-eGFP cohort (Controls) (n = 6) both as total and phosphorylated levels (*p < 0.05; **p < 0.01 non parametric Mann-Whitney test). (d) Oligomeric/aggregated tau was measured using the DA9/DA9 mono-ELISA: while a trend towards reduction was found across the cortex and the HB, the scFvMC1 treated mice (n = 6) exhibited a significant decrease of oligomeric tau in the hippocampus (*p = 0.0303, non parametric Mann-Whitney test) compared to the controls (n = 6). Graphs are expressed as % Control, and means +/− SEM