Fig. 7

Immunolocalization of BAG3 and HSPB7 in muscle fibers of adult wildtype and Hom animals. a In WT fibers BAG3 colocalizes together with FLNc in myofibrillar lesions and Z-discs identified with an antibody against an epitope of titin close to the Z-disc. In Hom muscle fibers, BAG3 localizes to Z-discs, but not to lesions. b In WT fibers also HSPB7 colocalizes with FLNc in myofibrillar lesions and Z-discs. In Hom muscle fibers, the small amount of HSPB7 that is expressed mainly localizes to lesions. Note that in all cases, identical scan settings were applied. For all experiments the RR90 antibody was used to detect FLNc. Bars: 5 μm