Fig. 4

Proteomic profiling of DIPG foci. a-b. WB analysis with indicated antibodies of total protein lysates (50 μg proteins) from tumor (T) and normal (N) fresh frozen autopsy samples. Primary pontine (P) foci are indicated in red font and secondary cerebellar (C) and frontal (Fr) foci, in green font. Sample laterality: R, right; L, left. Supplemental Fig. S6 shows the positive control for H3 K27Me3 antibody from an adult glioblastoma autopsy case with EZH2 overexpression. Note lack of H3 K27 methylation in the presence of EZH2 overexpression for F12 right frontal (RFr) tumor focus. c. Heat map of semiquantitative WB analysis, as shown quantified and normalized to loading controls in Supplemental Fig. S6. Shades of red and blue indicate expression level increase or decrease, respectively, as compared to normal controls