PrP Substrate | Reaction buffer | Parameters | Cohort size (N)a | Sens. | Spec. | Ref. |
---|---|---|---|---|---|---|
Hu 23–231 | 0.06–0.1 g/L substrate, 15 μL seed, 500 mM NaCl | 30 s circular shaking, 30 s incubation, 2 min break, 37 °C | 18 def sCJD | 83% |  | [5] |
35 def non-CJD | Â | 100% | ||||
25 prob. sCJD | 100% | Â | ||||
130 ctrl | Â | 100% | ||||
16 prob. sCJD | 87% | Â | ||||
14 ctrl | Â | 100% | ||||
0.05 g/L substrate, 5 μL seed, 500 mM NaCl | 24 gCJD | 83% | - | [81] | ||
20 GSS | 90% | - | ||||
12 FFI | 83% | - | ||||
Ha 23–231 | 0.1 g/L substrate, 15 μl seed, 300 mM NaCl | 1 min double orbital shaking 600 rpm, 1 min rest, 42 °C | cohort 1: 56 def sCJD | 91% |  | [54] |
52 ctrl | Â | 98% | ||||
cohort 2: 67 def sCJD | 87% | Â | Â | |||
52 ctrl | Â | 100% | Â | |||
15/13 def/prob. sCJD | 79% | Â | [59] | |||
43 ctrl | Â | 100% | ||||
179/97/29 def/prob./pos sCJD | 81% | Â | [50] | |||
46 gCJD | 91% | Â | ||||
348 ctrl | Â | 99% | ||||
24/26 def/prob. sCJD | 73% | – | [13] | |||
Ha 14–128 - Sheep 141–234 (chimera) | 0.1 g/L substrate, 15 μL seed, 170 mM NaCl | 1 min double orbital shaking, 600 rpm, 1 min rest, 42 °C | 64 def sCJD | 80% |  | [22] |
39 gCJD | 100% | Â | ||||
400 ctrl | Â | 99% | ||||
Ha 90–231 | 0.1 g/L substrate, 15–30 μL seed, 300 mM NaCl, 0,002% SDS | 1 min double orbital shaking 700 rpm, 1 min rest, 55 °C | 10/1 def/prob. sCJD | 91% | - | [60] |
43/5 def/prob. sCJD | 96% | Â | ||||
39 ctrl | Â | 100% | ||||
36/68 def/prob. sCJD | 94% | Â | [36] | |||
64 ctrl | Â | 100% | ||||
111 sCJD | 92% | Â | Â | |||
15 gCJD | 93% | Â | [30] | |||
67 ctrl | Â | 98.5% | Â | |||
63 sCJD, 14 ctrlb | 95% | 100% | Â | |||
10/12 def/prob. sCJD | 95% | Â | [13] | |||
17 ctrl | Â | 100% | ||||
116/73 def/prob. sCJD | 93% | Â | [31] | |||
33 gCJD | 100% | Â | ||||
100 ctrl | Â | 100% | ||||
61/41 def/prob. sCJD | 96% | Â | [29] | |||
80 ctrl | Â | 100% | ||||
439 def sCJD | 93 | Â | [72] | |||
31 gCJD | 97% | Â | ||||
69 ctrl | Â | 98.5% |