Fig. 2
Main fields of application of the RT-QuIC assay and kinetic parameters analysed. Prion disease (a, b): spongiform change (a) and perivacuolar pattern of PrPSc deposition (b) in the occipital cortex of sCJD MM2C. Synucleinopathies (c, d): Lewy bodies and neurites in the substantia nigra of a DLB patient (c); glial cytoplasmic inclusions in the cerebellar with matter of a MSA case (d). 4R-tauopathies (e, f): tufted astrocyte (e), and astrocytic plaque (f) in the striatum of PSP and CBD cases. Hematoxylin and eosin stain (a), immunohistochemistry for PrP (mAb 3F4, dilution 1:400, Signet Labs, (b), α-Syn (LB509, dilution 1:100, Thermo Fisher, (c, d), hyperphosphorylated tau (AT8, dilution 1:100, Innogenetics), (e, f). Lag phase is defined as the time interval between the beginning of the reaction and the time in which the curve of the fluorescent signal crosses the threshold (dot line); ThT max (circle) is defined as the maximum fluorescence value reached by the curve; AUC (area under the curve)