Fig. 1

Schematic representation of the RT-QuIC reaction. The RT-QuIC reaction may be divided into temporal stages: the lag phase, the exponential phase and the plateau phase. The lag phase represents the time required for the reaction to take place. During this step, the seed is allowed to contact the substrate and to trigger the conformational change. Once enough energy is received by the system, ThT sensitive oligomers emerge, which incorporate the monomers into small aggregates (exponential phase). Eventually, when all the substrate is incorporated into fibrils, a plateau phase is observed