Table 1 Tested 5-Lox and FLAP antibodies on mouse brain tissue and their immunoreactivity in neurons and/or microglia cells. aa = amino acid; − = no, + = mild, ++ = moderate and +++ = strong immunoreactivity
Antibody (host species) | Dilution | Reactivity | Epitope/antigen | Neurons | Microglia | Others |
|---|---|---|---|---|---|---|
polyclonal Abcam #ab39347 (rabbit) | 1:100 | mouse, rat, hamster, human, pig | human 5-Lox aa. 130–149 | ++ | ++ | + |
monoclonal Abcam #ab169755 (rabbit) | 1:100 | mouse, rat, human | human 5-Lox aa. 100–200 | ++ | + | – |
monoclonal BD Biosciences #610694 (mouse) | 1:50 | chicken, human, rat, mouse | human 5-Lox aa. 442–590 | +++ | – | – |
polyclonal Novus Biologicals NBP1–84666 (rabbit) | 1:100 | human, rat | recombinant protein | – | +++ | – |
polyclonal Novus Biologicals NB300–891 (goat) | 1:150 | human, mouse, rat | peptide corresponding to human FLAP (C-terminus) | – | ++ | – |