Fig. 3

Immunohistochemical analysis for 5-Lox and FLAP expression in WT and APP-PS1 mouse brains. a In mouse brains the majority of 5-Lox (green, monoclonal 5-Lox antibody from Abcam #ab169755) immunoreactivity was observed in the granular layer of the dentate gyrus and co-localized with NeuN (white). b 5-Lox immunoreactivity (green, using polyclonal 5-Lox antibody from Abcam #ab39347) was also observed outside the granular layer in particular in APP-PS1 mice and co-localized with some Iba1 positive microglia (white) cells of WT and APP-PS1 transgenic mice (inserts). c Besides co-expression of 5-Lox (green, using polyclonal 5-Lox antibody from Abcam #ab39347) in some microglia cells, also GFAP (red) positive astrocytes showed immunoreactivity for 5-Lox in WT and APP-PS1 mice (inserts). d FLAP (green, polyclonal FLAP antibody from Novus Biologicals #NBP1–84666) was ubiquitously expressed in Iba1 positive microglia cells (white) in WT and APP-PS1 transgenic mice. Dapi (a-d) was used as nucleus stain. Scale: 20 μm (b-d right images), 50 μm (a-d left images)