Fig. 6

Equalized microglial Aβ phagocytosis in the hippocampus of aged 5xFAD mice. (a) Representative immunofluorescence images of TR (red) and Iba1 (green) on coronal hippocampal sections from 10 months old 5xFAD mice. Nuclei were stained with DAPI (blue). Scale bar: 50 μm. Quantification of (b) Iba1⁺ parenchymal in hippocampus of 5xFAD and age-matched WT mice. Quantification of (c) TR⁺ plaque-associated microglia in 5xFAD mice. Each symbol represents one mouse. Data are presented as mean ± s.e.m. Significant differences were determined by two-way ANOVA followed by Bonferroni post-hoc comparison test (**P < 0.01, ***P < 0.001). (d) Gating of CD11b⁺methoxy-XO4⁺microglia from SPF, GF and ABX-treated 5xFAD mice and age-matched WT controls. Representative flow cytometric dot plots are shown. (e) Representative cytometric graph of methoxy-X-O4⁺ labelled microglia from SPF (black line), GF (red line) and ABX-treated (blue line) 5xFAD mice and respective WT controls (dashed lines). (f) Quantification of percentages of methoxy-X-O4⁺ labelled microglia cells are depicted. Each symbol represents one mouse. Data are presented as mean ± s.e.m.: No significant differences were detected by two-way ANOVA followed by Bonferroni post-hoc comparison test. Data are representative of three independent experiments