Fig. 8

Nicotinamide (NAM) rescues axon defects of Tdp-43 knockdown motoneurons. a Scatter plot of axon lengths of Tdp-43 knockdown (shTdp-43) and control motoneurons cultured in the absence (−) or presence of NAM at the indicated concentrations. Horizontal lines represent median. ****p ≤ 0.0001; Kruskal-Wallis test with Dunn’s multiple comparisons test (N numbers of axons are shown in the graph, n = 3 independent experiments). b Immunostaining of control and Tdp-43 knockdown motoneurons with antibodies against puromycin and Tau. Motoneurons were transduced with control or Tdp-43 knockdown lentivirus and cultured in the presence (+) or absence (−) of 0.1 mM NAM. Puromycin labeling was performed at DIV6. Scale bar: 20 μm. c Scatter plot showing the axon-to-soma ratios of puromycin intensity in control and Tdp-43 knockdown motoneurons. Horizontal lines represent median. *p ≤ 0.05, ****p ≤ 0.0001; Kruskal Wallis test with Dunn’s multiple comparisons test (N numbers of motoneurons are shown in the graph, n = 3 independent experiments)