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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Loss of Tdp-43 disrupts the axonal transcriptome of motoneurons accompanied by impaired axonal translation and mitochondria function

Fig. 7

Mitochondrial dysfunction in axons of Tdp-43 knockdown motoneurons. a Left panel: Axons of control and Tdp-43 knockdown (shTdp-43) motoneurons stained with MitoTracker™ Orange CM-H2TMRos. Scale bar: 5 μm. Right panel: Scatter plot depicting the number of mitochondria stained with MitoTracker™ Orange CM-H2TMRos normalized to axon length. Horizontal lines represent median. **p ≤ 0.01; Mann-Whitney test (N = 36 axons for control and 32 axons for Tdp-43 knockdown motoneurons, n = 2 independent experiments). b Left panel: Motoneurons transduced with control lentivirus (expressing GFP) were labeled with TMRM for live-cell imaging. Cells were treated with FCCP at the indicated timepoint to disrupt the mitochondrial membrane potential. Scale bar: 20 μm. Right panel: Quantification of TMRM intensity in mitochondria with time. Data represent mean ± SD (n = 5 mitochondria). c Left panel: Axons of control and Tdp-43 knockdown motoneurons labeled with TMRM. Scale bar: 5 μm. Right panel: Quantification of TMRM mean intensity of mitochondria in axons of control and Tdp-43 knockdown motoneurons. Horizontal lines represent median. **p ≤ 0.01; Mann-Whitney test (N = 25 axons for control and 38 axons for Tdp-43 knockdown motoneurons, n = 3 independent experiments)

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