Fig. 5

Co-labelling of p-PERK and p-eIF2α with tau in AD cases. (a-d) Fluorescent triple labelling of p-PERK and p-eIF2α with NeuN and tau in the AON (a-b) and CA1 region (c-d). NeuN+ cells that contained p-PERK or p-eIF2α and tau (arrowheads) often had diffuse tau aggregates rather than dense neurofibrillary tangles. (e-f) Stacked bar graph indicating the percentages of p-PERK+ cells (e) or p-eIF2α + cells (fα) that did or did not contain tau. A low percentage of p-PERK+ or p-eIF2α + cells contained tau and this was not significantly different across the olfactory or hippocampal sub-regions we assessed. (g-h) Stacked bar graph indicating the percentages of tau+ cells that did or did not contain p-PERK (g) or p-eIF2α (h). A very low percentage of tau+ cells contained p-PERK or p-eIF2α and this was significantly higher in the CA1 region compared to the AONi and EC for p-PERK, and the AONi alone for p-eIF2α. Scale bars = 50 μm