Fig. 2

Distribution of p-PERK and p-eIF2α in the human AD olfactory bulb relative to tau and beta-amyloid aggregates. a Representative sagittal section of a human AD olfactory bulb labelled for tau, beta-amyloid, and PGP9.5 with Hoechst (nuclei) stain, indicating the location of aggregates within the anterior olfactory nucleus (AON) and the mitral cell layer/ external plexiform layer (MCL/EPL). b A schematic diagram of the location of markers overlaid on the same sagittal section. The outline of the olfactory bulb is drawn in dark grey. The glomeruli and the AON have been traced in light grey based on PGP9.5 immunoreactivity. The locations of p-PERK+ cells are indicated by green dots and the locations of p-eIF2α + cells on an adjacent section have been indicated by red dots. p-PERK+ and p-eIF2α + cells were predominantly located in the AON and MCL/EPL. Neurons containing p-PERK (C-G, arrows) and p-eIF2α (H-L, arrows) were identified in all the olfactory regions investigated in this study. Abbreviations: CA1, Cornu Ammonis 1; EC, entorhinal cortex; GCL, granule cell layer; GL, glomerular layer; PIR, piriform cortex. Scale bars = 500 μm (A-B), 20 μm (C-L)