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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Erythrocytic α-synuclein contained in microvesicles regulates astrocytic glutamate homeostasis: a new perspective on Parkinson’s disease pathogenesis

Fig. 5

Astrocytes take up RBC-EVs via dynamin-dependent endocytosis resulting in dysfunction of glutamate clearance. a Representative images of cultured astrocytes treated with DiI-labeled RBC-EVs in the presence or in the absence of dynasore or EiPA. Application of dynasore prevented astrocyte uptake of RBC-EVs (Scale bar, 10 μm). b Quantification analysis of percentage of astrocytes showing RBC-EV uptake (means + S.E.M; n = 3; *p < 0.05, ****p < 0.0001 by One-way ANOVA followed by Tukey-Kramer’s post-hoc test). c Quantification analysis of uptake of H3-labelled aspartic acid in astrocytes. The presence of dynasore mitigated the effect of RBC-EVs (means + S.E.M; n = 3; ***p < 0.001 by ordinary Two-way ANOVA test and Sidak’s multiple comparisons post-test)

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