Fig. 6

AAV-tau^P301L did not cause overt neuronal cell loss at 6 months of age. a Crude brain lysates, probed by western blot, showed no change in NeuN between all mice groups. However, PSD95 was significantly decreased in WT-AAV mice. b Left: Quantification of NeuN showed no difference between the 4 groups (SI Table 1). Right: Quantification of PSD95 showed a 39.1% decrease in PSD95 in WT-AAV relative to WT uninjected (p = 0.0049). There was no difference between WT uninjected and Fyn KO uninjected mice (p = 0.9999) nor between WT uninjected and Fyn KO-AAV (p = 0.9073). For NeuN, 8 WT, 9 WT-AAV, 9 Fyn KO, and 9 Fyn KO-AAV were used. For PSD95, 7 WT, 9 WT-AAV, 9 Fyn KO, and 8 Fyn KO-AAV were used. c) Quantification of NeuN positive cells showed no difference between the four groups in Ammon’s horn (p = 0.5214). 3 WT, 3 WT-AAV, 3 Fyn KO, and 4 Fyn KO-AAV mice were examined. Two-way ANOVA with Tukey’s post hoc multiple comparisons were used for all panels. Statistical results are shown in SI Table 1