Fig. 5

Both Fyn depletion and tau^P301L caused behavior abnormality. a In the open field test, no difference in the total number of movements was detected between tau^P301L injected or uninjected WT and Fyn KO mice (SI Table 1). 8 WT, 21 WT-AAV, 10 Fyn KO, 8 Fyn KO-AAV mice were used. b Uninjected WT mice spent more time in the center of the open field apparatus relative to the uninjected Fyn KO and Fyn KO-AAV (p = 0.0002 and p = 0.0049, respectively). Fyn KO-AAV mice were similar to uninjected Fyn KO (p = 0.94). If t-test instead of two-way ANOVA was used, uninjected WT spent significantly more time in the center of the field than WT-AAV (t-test p = 0.0333). c In the elevated plus maze, the four groups of mice did not behave significantly different, as evaluated by two-way ANOVA (SI Table 1). However, if t-test were used instead, uninjected WT differed significantly from WT-AAV (t-test p = 0.0342) whereas uninjected Fyn KO did not differ from Fyn KO-AAV (t-test p = 0.7203); uninjected Fyn KO was significantly different from uninjected WT (t-test p = 0.0022). 15 WT, 18 WT-AAV, 10 Fyn KO, and 8 Fyn KO-AAV were used. d Relative to uninjected WT, WT-AAV mice had learning deficits during training day of contextual fear conditioning (day 1). Relative to uninjected Fyn KO, Fyn KO-AAV mice did not have any learning disabilities. Asterisks indicate statistical comparisons to WT uninjected mice. 12 WT, 20 WT-AAV, 8 Fyn KO, and 9 Fyn KO-AAV were used. e Relative to uninjected WT, WT-AAV mice displayed memory deficits in the testing day of contextual fear conditioning (day 2) while Fyn KO-AAV mice did not, relative to Fyn KO uninjected mice. However, both Fyn KO and Fyn KO-AAV displayed memory deficits relative to WT uninjected and WT-AAV mice. Asterisks indicate comparisons to WT uninjected mice; Fyn KO and Fyn KO-AAV mice shared the same p-values when compared to WT. Significant differences were found between WT-AAV and Fyn KO/Fyn KO-AAV at times 2,3,4,5, and 6 min (p values are in SI Table 1). For d and e, * denotes p ≤ 0.05, ** denotes p ≤ 0.01, *** denotes p ≤ 0.001, ****denotes p ≤ 0.0001. Two-way ANOVA with Tukey’s post hoc multiple comparisons were used for all panels. Statistical results are shown in SI Table 1