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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Fyn depletion ameliorates tauP301L-induced neuropathology

Fig. 1

Fyn KO-AAV mice displayed less Bielschowsky silver stained and Thioflavin-S positive NFTs than WT-AAV mice. a, b, c, d Hippocampus of WT mice (a, c) or Fyn KO mice (b, d), injected with AAV2/8-tauP301L, were subjected to Bielschowsky silver staining 6 months post injection. Fyn KO-AAV showed less NFTs than WT-AVV. a, b Scale bar: 200 μm. c and d showed enlarged portions from panels a and b, respectively; scale bar: 50 μm. e Bielschowsky stained tangles were quantitated as described in Methods; three fields from each of six animals was examined. **p = 0.0187, as calculated by t-test. f, g, h, i Hippocampus of WT mice (f, h) or Fyn KO mice (g, i), injected with AAV2/8-tauP301L, were subjected to Thioflavin S staining 6 months post injection. Fyn KO-AAV showed less NFTs than WT-AVV. g, i Scale bar: 200 μm. h and i showed enlarged portions from panels f and g, respectively; scale bar 50 μm. 3 WT-AAV and 3 Fyn KO-AAV mice were used and representative images were shown. Sections from the same mice were used for panels a and f and for panels b and g. While all WT-AAV mice examined contained minimal pathology in the dentate gyrus area, one of three Fyn KO-AAV mice examined had clear Bielschowsky silver stained filaments in the dentate gyrus area and thioflavin S positive staining (b, g, SI Fig. 4). However, Cook et al., who had also used P0 injection of AAV-tauP301L to create a tauopathy model in WT mice, reported pathology in the dentate gyrus (Fig. 1j in [14]). Therefore, the pathology in the dentate gyrus of the Fyn KO-AAV was not unique to the Fyn KO genotype. The variability between our findings and those of Cook et al. indicated that comparisons would best be made within an experimental system, since between laboratories, the level of tau expression may vary, in part depending on AAV preparation used and virus titer injected

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