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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: A novel AFG3L2 mutation close to AAA domain leads to aberrant OMA1 and OPA1 processing in a family with optic atrophy

Fig. 2

Overexpression of exogenous AFG3L2G337E-myc in a AFG3L2 null background does not rescue L-OPA1 and mitochondrial tubulation. a WB analysis and relative quantification of L-OPA1 after transient transfection of mt-YFP in combination with AFG3L2 WT-myc or AFG3L2G337E-myc in Afg3l2+/+ and Afg3l2−/− MEFs (ratio 1:3). c-MYC was used as transfection control. Bars represent means ± SEM of three independent experiments. Student’s t test: * p < 0.05; ** p < 0.01; *** p < 0.001. b Representative pictures of in live mitochondrial morphology after transient transfection of mt-YFP in combination with AFG3L2 WT-myc or AFG3L2G337E-myc in Afg3l2+/+ and Afg3l2−/− MEFs (ratio 1:3). The graph shows the morphometric analysis of mitochondrial morphology. At least 80 randomly selected cells were analyzed in each experiment. Chi-square analysis (two degrees of freedom): *** p < 0.001

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