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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation

Fig. 6

Rescue of neuronal migration defects by the recruitment of BicD2 K775X to the NE. a Schematic diagram of the HA-BicD2 K775X protein fused with KASH domain. b Expression of HA-BicD2 K775X-KASH in HEK293T cells transfected with the construct. c Subcellular localization of HA-BicD2 K775X-KASH (green) in cultured HeLa cells. The protein showed prominent NE localization. Cells were stained with DAPI (blue) to show the cell nuclei. Bar = 5 μm. d Coronal sections of mouse brains collected 4 days after electroporation of BicD2 WT, K775X and K775X-KASH at E14.5. Fusion of the KASH domain to BicD2-K775X rescued neuronal migration defects caused by BicD2 K775X mutation. All slices were stained with DAPI (blue) to show the cell nuclei. Bar = 100 μm. e Bar graph with individual data points showing cell distribution in the CP, IZ, and VZ 4 days after electroporation (n = 3 pregnant females in each condition). Error bars represent SEM. *P < 0.05, **P < 0.01. ANOVA. Post hoc: Bonferroni test

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