Fig. 3

Differentiation of cells expressing BicD2 WT and K775X into cortical neurons postnatally. a Expression of neuronal marker NeuN (red) in brains electroporated with BicD2 WT or K775X (green). Coronal sections of the mouse brains were collected at P7 after in utero electroporation at E14.5. The majority of cells electroporated with BicD2 WT or K775X were NeuN+, even though K775X-expressing cells were arrested in the WM. Bar = 100 μm in the top panel, bar = 25 μm in the lower panel. Bar graph with individual data points shows the percentage of NeuN+/GFP+ cells in the electroporated brain slices (n = 3 mice from 3 independent pregnancies). Error bars represent SEM. Student’s t test. b Expression of the marker for cortical layer II-IV, Cux1, in brains electroporated with BicD2 WT or K775X (green). Again, most of the electroporated cells were Cux1+ in both groups. All slices were stained with DAPI (blue) to show the cell nuclei. Boxed regions were shown at a higher magnification below each panel. Bar graph with individual data points shows the percentage of Cux1+/GFP+ cells in the electroporated brain slices (n = 3 mice from 3 independent pregnancies). Error bars represent SEM. Student’s t test