Fig. 3

Characterization of the specificity of a new monoclonal antibody 3G12 against pSer208. a ELISA was performed to assess the specificity of 3G12 monoclonal antibody compared to AT8 and PBS controls. Peptides used in ELISA are shown in Table 1. b Brain Triton X-100 insoluble fractions from tau knockout mice (tau KO), nontransgenic mice (nTG), and 6 month old rTg4510 tau transgenic mice were loaded on 10% polyacrylamide gels for immunoblotting analysis. Membranes were probed with (b) 3G12 monoclonal antibody or (c) rabbit polyclonal antibody 3026. The relative molecular masses of protein markers are indicated on the left