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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: A novel mechanism of phenotypic heterogeneity in Creutzfeldt-Jakob disease

Fig. 4

Relative abundance of 129 M and 129 V resPrPD purified from distinct variants of sCJDMV. Relative proportions of both allotypic proteins in each case were determined by mass spectrometry using the spectral counting method. For the histotypically pure MV1, MV2C and MV2K variants data ± SEM are averages of three determinations for each of the nine total cases. For the mixed cases MV2K-C (~ 50:50) and MV2K-C 0:100 K:C in the cerebral and ~ 90:10 K:C in the cerebellar cortices, data are averages of three MS determination for each case. The fractions (%) of resPrPD-129 M in each MV2 variant and the MV1 subtype are: 83 ± 7% for MV2C, 23 ± 7% for MV2K, and 48 ± 2% for MV1. In pure MV2C and MV2K cases, the relative amounts of resPrPD-129 M and 129 V are significantly different (p < 0.0001 and 0.0002, respectively) whereas in MV1 the difference is not significant. In the two MV2K-C mixed cases, the resPrPD-129 M proportion is 44% for the CC ~ 50:50 first case; 78 and 40%, respectively, for the CC 0:100 and CbC ~ 90:10. As determined by one way ANOVA, p values for differences in relative populations of the two resPrPD allotypic forms in distinct sCJD variants and subtypes are: MV2C vs. MV2K, p < 0.0001; MV1 vs. MV2C p  <0.009

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