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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases

Fig. 1

RBM45 association with membraneless nuclear organelles. a-c HEK293 cells were immunostained for endogenous RBM45 and marker proteins of the indicated organelles. Under basal conditions, RBM45 is diffusely localized throughout the nucleus and does not associate with nuclear speckles, Cajal bodies, or nuclear gems (a-c, respectively). d Sodium arsenite (0.5 mM for 1 h) was used to induce cytoplasmic stress granule formation. RBM45 does not associate with G3BP1-positive cytoplasmic stress granules during cellular stress, but coalesces into nuclear puncta. e Heat shock (42 °C for 1 h) led to a redistribution of SAFB (top) and HSF1 (bottom) into nuclear stress bodies (NSBs). Stress-induced RBM45 nuclear foci correspond to NSBs, indicated by co-localization of RBM45 with the NSBs. For all images, scale bar = 5 μm

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