Fig. 4From: A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse gliomanCATS can simultaneously quantify MGMT CpG methylation and detect Single nucleotide variants (SNVs) in glioma clinical samples. a,MGMT gene expression in 4 IDH wild type samples by qRT-PCR. Data are the mean ± SD (3 technical replicates). b, Methylation pattern by nCATS and MassARRAY. c, Correlation between MGMT expression and exon 1 methylation and between MGMT expression and intron 1 methylation. d, SNVs in MGMT and IDH1/2 were assayed with nCATS and Illumina sequencing in tumor and saliva samples from 6 patients. Data were plotted with trackViewer. No data were available for P785 and P816Back to article page