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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse glioma

Fig. 4

nCATS can simultaneously quantify MGMT CpG methylation and detect Single nucleotide variants (SNVs) in glioma clinical samples. a,MGMT gene expression in 4 IDH wild type samples by qRT-PCR. Data are the mean ± SD (3 technical replicates). b, Methylation pattern by nCATS and MassARRAY. c, Correlation between MGMT expression and exon 1 methylation and between MGMT expression and intron 1 methylation. d, SNVs in MGMT and IDH1/2 were assayed with nCATS and Illumina sequencing in tumor and saliva samples from 6 patients. Data were plotted with trackViewer. No data were available for P785 and P816

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