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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: A novel Cas9-targeted long-read assay for simultaneous detection of IDH1/2 mutations and clinically relevant MGMT methylation in fresh biopsies of diffuse glioma

Fig. 3

Correlation between MGMT gene expression and CpG methylation at different loci. a,MGMT gene expression was measured with qRT-PCR in 4 cell lines and 4 IDH-mutant tumor samples. Data are the mean ± SD (3 technical replicates). b, Percent methylation of 12 clinically relevant CpG sites within MGMT exon 1. c, Correlation between MGMT expression and methylation detected by pyrosequencing vs. nCATS. Each yellow point is an individual sample. d, Heat map and hierarchical clustering of percent methylation of the exon 1 CpGs and a portion of the intron 1 CpGs. Selected CpGs (r > 0.7 or r < − 0.7) were used for clustering. e, Correlation between MGMT expression and exon 1 methylation and between MGMT expression and intron 1 methylation

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