Quantitative patterns of motor cortex proteinopathy across ALS genotypes

Table 2 Primary antibody clones used in this study

Antigen	Supplier	Catalogue #	Host	Clonality	Dilution
Antigen	Supplier	Catalogue #	Host	Clonality	IHC	MxIF
pTDP-43^a	Cosmo-bio	TIP-PTD-M01	Mouse	Monoclonal	1:40000	1:10000
p62^b	Abcam	ab91526	Rabbit	Polyclonal	1:1000	–
FUS^c	Sigma-Aldrich	HPA008784	Rabbit	Polyclonal	1:300	–
SOD1^d	StressMarq Bio	SPC-206	Rabbit	Polyclonal	1:1000	–
HuC/D^e	Santa Cruz	sc-28,299	Mouse	Monoclonal	–	1:400
SMI312^f	Sternberger monoclonals	837,904	Mouse	Monoclonal	1:2000	–
NeuN	Abcam	ab177487	Rabbit	Monoclonal	1:2000	–
Parvalbumin	Swant	235	Mouse	Monoclonal	1:7500	1:5000
GFAP^g	Sigma-Aldrich	C9205	Mouse	Monoclonal	–	1:800
Olig2^h	Abcam	ab109186	Rabbit	Monoclonal	1:400	–
TPPP/p25ⁱ	Win-Fest Ltd	–	Mouse	Monoclonal	1:2000	–
Iba1^j	WAKO	019–19,741	Rabbit	Polyclonal	–	1:400
CD68^k	Dako	M0876	Mouse	Monoclonal	1:200	–
CD68^k	ThermoFisher	MS397	Mouse	Monoclonal	–	1:800
Collagen IV	Merck Millipore	MAB3326	Mouse	Monoclonal	–	1:4000
OPTN^l	Proteintech	10,837–1-AP	Rabbit	Polyclonal	1:3000	–
GAD67^m	Merck Millipore	MAB5406	Rabbit	Monoclonal	1:1000	–

^apTDP-43 - Phosphorylated transactive DNA response protein 43 kDa; ^bp62 – Sequestosome; ^cFUS – Fused-in-sarcoma; ^dSOD1 – Superoxide dismutase 1; ^eHuC/D – ELAV-like protein 4; ^fSMI312 – Panaxonal Neurofilament marker; ^gGFAP – Glial fibrillary acidic protein; ^hOlig2 – Oligodendrocyte transcription factor 2; ⁱTPPP – Tubulin polymerization-promoting protein; ^jIba1 – Ionized calcium-binding adapter molecule 1; ^kCD68 – Cluster of differentiation 68; ^lOPTN – Optineurin; ^mGAD67 – Glutamate decarboxylase 67

ISSN: 2051-5960