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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Quantitative patterns of motor cortex proteinopathy across ALS genotypes

Fig. 5

Cortical inhibitory interneurons rarely accumulate pTDP-43. Motor cortex GAD67+ neurons were present predominantly within layers II-V (a), and exhibited varied morphologies including large neurons with long apical dendrites (b, green arrow), smaller tufted neurons (b, blue arrow). GAD67 highlights multiple primary and secondary dendrites (c). GAD67 interneurons were seen synapsing directly on to Betz cell somata (d, green arrow). GAD67 neurons were devoid of granular or compact pTDP-43 aggregates, even in areas that otherwise contained typical ALS-TDP neuropathology (e-g; orange arrows: pTDP-43 in pyramidal cells, green arrow: lack of pTDP-43 in a Betz cell (5e) and non-Betz pyramidal cell (5 g)). We found only one instance of clear cytoplasmic colocalization of GAD67 and a compact pTDP-43 aggregate (5 h, green arrow). Multiplexed IHC revealed no evidence of parvalbumin colocalising with pTDP-43 (i, green arrow indicates HuD+, parvalbumin- neuron containing discrete pTDP-43 inclusion). Scale bars (μm): a = 300; b = 30; c,g = 20; d,e,f = 25; h = 15; i = 50

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