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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Quantitative patterns of motor cortex proteinopathy across ALS genotypes

Fig. 1

Quantitative assessment of pathology in the ALS primary motor cortex and spinal cord using optimised digital image analysis. IHC staining was quantified in the motor cortex using DAB optical-density based algorithms on five ROI, each measuring 3mm2. ROI were spread evenly around a single gyrus where the top edge of the ROI was at the pial surface (a). CD68 and pTDP-43 pathology was also quantified in the anterior horn and corticospinal tract of the lumbar spinal cord (b, yellow and green box respectively), using guidelines bisecting the section in each direction across the central canal. An optimised positive pixel count (ppc) algorithm is able to distinguish variable pTDP-43 morphologies including glial (c, red arrows) and neuronal (d, red arrows). A positive cell count (pcc) algorithm counts the number of Olig2+ oligodendrocytes (e, black box = area of f), and defines positive cells by DAB-threshold with high specificity (f, pink arrow highlights DAB-positive cell, green arrow highlights DAB-negative cell). Scale bars where not indicated (μm): c,d = 50; e = 250; f = 20

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