Fig. 5

Post-MRI pathology shows iNSC reduction of astrogliosis. a-e: Coronal sections from chronic CPZ mice with vehicle (Veh) or iNSC injection immunolabeled to detect myelin (MOG; a-b), or the innate immune response of astrocytes (GFAP; c-d) or microglia (IBA1; e) with DAPI nuclear stain (blue; c-e). Panel C shows an example of transplanted iNSCs (green, GFP) in the CC and adjacent white matter (cingulum; Cg) and gray matter regions (cortex, Cx; hippocampus, Hp). Panel D shows higher magnification of iNSCs (green, GFP) in the CC with examples of round cells (white arrows) and elongated cells with extended processes (yellow arrow). Panel E shows IBA1 immunolabeling of microglia (red) with lipofuscin granules (yellow autofluorescence) that accumulate after chronic CPZ. f: Coronal CC sections processed for in situ hybridization to detect oligodendrocytes expressing proteolipid protein (Plp1). g-k: Quantification of tissue analysis in this post-MRI cohort shows CC atrophy and demyelination are not attenuated by iNSCs (g, h). Transplanted iNSCs reduced astrogliosis (i), but did not change the microglial (j) or oligodendrocyte response (k). Bar color reflects condition as chronic cuprizone followed by vehicle injection (black) or iNSC transplant (green). Data are mean values ± sem. Vehicle (n = 5) and iNSC (n = 6 IHC, n = 5 Plp1) conditions were compared using t-tests. Scale bars: A, B shown in A = 200 μm; C = 200 μm; D = 20 μm; E = 100 μm; F = 40 μm