Fig. 4

MRI radial diffusivity indicates reduced CC pathology with iNSC transplantation. a: Experimental timeline for longitudinal imaging of mice across 4 time points: baseline (BL) before initiating CPZ, at 6 weeks of CPZ, and at 12 weeks of CPZ before and finally at 2 weeks after iNSC, or vehicle, injection. After the scan at 6 weeks of CPZ, mice were randomized to vehicle or iNSC injection. After 12 weeks of CPZ, mice were returned to normal chow pellets for 1 day before intracerebral injection. CC region-of-interest (ROI) for quantification shown in yellow with iNSC injection path shown in green, with DAPI nuclear staining of cytoarchitecture. b: Representative T2-weighted and DEC images. Arrows indicate medial CC regions that show CPZ effect. Colors reflect fiber directions as red (medial-lateral), blue (anterior-posterior), and green (superior-inferior). c-h: Quantitative analysis of the CC shows significant differences from baseline result from CPZ administration using values from T2-weighted imaging (c), MTR (d), trace (e), FA (f), and RD (h). The AD values only differ from baseline at the 14 week time point (g). The comparison of iNSC transplantation versus vehicle (Veh) shows a significant effect in RD values (h, green bracket). Horizontal line shown extends from the baseline value of the cohort that later received iNSCs after 12 weeks of CPZ. Bar color reflects condition as baseline (gray fill), demyelinated with CPZ (black fill) or iNSC transplant after chronic CPZ (green fill). Borders around bars denote same mice that received iNSC (green border) or vehicle (black border) after ending cuprizone. Data are mean values ± sem. Two-way ANOVA with repeated measures and Bonferroni multiple comparisons test for CPZ with injection of vehicle (n = 5) versus iNSCs (n = 6)