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Fig. 11 | Acta Neuropathologica Communications

Fig. 11

From: Transplantation of induced neural stem cells (iNSCs) into chronically demyelinated corpus callosum ameliorates motor deficits

Fig. 11

iNSC transplantation modulates axon damage and cycling OPCs after CPZ chronic demyelination in the cingulum. a-b: Tissues from mice with Miss-step wheel access during remyelination were further analyzed for axon damage in the cingulum to quantify the separate axon population in each hemisphere relative to vehicle and iNSC injections. Axon damage was detected by immunolabeling of total axons with neurofilament-H (a) and damaged axons with SMI32 for nonphosphorylated neurofilaments (b). Axon damage was increased significantly in chronic CPZ mice injected with vehicle while iNSC transplantation slightly improved axon health toward naïve values (b). c: Chronic CPZ increased overall cellularity in the cingulum, which was not altered by iNSC transplantation. d: Proliferating cells, identified by Ki67, were rare after chronic CPZ but increased with iNSC transplantation, which was largely due to an increase of NG2 immunolabeled OPCs in the ipsilateral (ipsi) cingulum. e-g: Representative images of each condition for neurofilament-H (pseudocolored green) labeled axons with SMI32 (red) co-labeling of damaged axons and DAPI (blue) nuclear stain. Two-way ANOVA with Sidak’s multiple comparisons test for naïve (n = 6), CPZ vehicle (n = 6), and CPZ iNSC (n = 6) comparison, and ipsi vs. contra comparison. Contingency table with Fisher’s exact test for comparison of Ki67 and NG2 in CPZ vehicle (n = 3) and CPZ iNSC (n = 3) mice. Data are mean values ± sem, except for contingency analysis that shows total counts for each condition. Scale bars E-G shown in E = 10 μm

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