Fig. 10

Transplanted iNSCs are localized mainly in white matter and express markers of oligodendrocyte and astrocyte differentiation. a: The relative distribution of iNSCs in neuroanatomical regions adjacent to the CC target site. iNSCs quantified by direct detection of GFP expression in tissue sections combined from analysis of mice in the MRI and behavior cohorts for neuropathology and iNSC cell type identification. b-d: Quantification of differentiation of transplanted iNSCs in white matter based on direct visualization of GFP expression and co-labeling with markers for neural stem/progenitor cells (Sox2), oligodendrocyte lineage cells (Olig2), or the astrocyte lineage (GFAP). Black fill shows counts of iNSC co-labeled for given cell marker, with upper bar section showing unlabeled iNSC counts. Percent co-labeling shown for each region. e-g: Examples of transplanted iNSCs expressing green fluorescent protein (GFP) along with nuclear Sox2 (e), nuclear Olig2 (f), or cytoplasmic GFAP (g). iNSC membranes labeled for GFP extend around cell bodies (pink arrows), along axons (white arrow), and to blood vessels (blue arrow). iNSCs were not identified as microglia in any sections analyzed with IBA1 immunolabeling. Microglia often contained autofluorescent lipofuscin granules (f, yellow arrows). Tissue sections from mice of both the MRI (n = 6) and behavior (n = 11) cohorts were combined for the analysis of iNSC distribution. Differentiation studies included a subset of mice (Sox2 (n = 6), Olig2 (n = 9), and GFAP (n = 14)) combined from MRI and behavior cohorts to generate contingency tables of total cell counts among sections analyzed for comparison using Fisher’s exact test. Scale bars E = 10 μm; F = 20 μm; G = 5 μm