Fig. 1

Mouse induced neural stem cells (iNSCs) exhibit stem cell characteristics in vitro. a-b: In vitro, iNSCs form neurospheres (a) and express NSC markers Sox2 (red) and Nestin (pseudocolored green). c-e: Following differentiation of iNSCs, constitutively expressed green fluorescent protein (GFP) is visible in all cells (c) while cells differentiated along an astrocytic lineage exhibit immunolabeling for GFAP (red, c). In addition, differentiation along neuronal (MAP2; red, d) and oligodendroglial (O4; red, e) lineages shows multipotency of iNSCs (GFP not shown). f: In non-differentiation conditions, iNSCs exhibit an exponential proliferation rate expected for stem cell growth based on quantification of each subculture after each passage. g: Quantification of iNSC multipotent differentiation from separately revived vials (n = 4). Data are mean values ± sem. Scale bar A = 100 μm, B, D, E = 20 μm, C = 40 μm