Skip to main content
Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Transmission characteristics of heterozygous cases of Creutzfeldt-Jakob disease with variable abnormal prion protein allotypes

Fig. 4

Type 1 PrPSc accumulates in the brains of tg66 and tgRM mice inoculated with heterozygous cases of MV2K sporadic and iatrogenic CJD. a PrPSc immunoblot of tg66 mice (left panel) and tgRM mice (right panel) inoculated with MV2K cases of sCJD. Type 1 PrPSc accumulates in all of the recipient mice. The numbers above the line indicate the case number while the numbers over the individual lanes represent the 3–4 brain samples that were analyzed for each CJD subtype inoculated. Lanes with no label above them are blank. b PrPSc immunoblot of tg66 mice (left panel) and tgRM mice (right panel) inoculated with MV2K cases of iCJD. Type 1 PrPSc accumulates in all of the recipient mice. The numbers above the line indicate the case number while the numbers over the individual lanes represent the 3–4 brain samples that were analyzed for each CJD subtype inoculated except for case 19 where only one sample was available for the tg66 mice. Blots were developed using the mouse monoclonal anti-PrP antibody 3F4 conjugated to biotin. Lanes marked MV1 and MV2 are from human cases of sCJD and represent the controls for Type 1 and Type 2 PrPSc, respectively. Molecular mass markers are indicated on the right side of the figure

Back to article page
\