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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Transmission characteristics of heterozygous cases of Creutzfeldt-Jakob disease with variable abnormal prion protein allotypes

Fig. 3

Type 1 PrPSc accumulates in the brains of most tg66 and tgRM mice inoculated with 3 different subtypes of MV heterozygous sCJD. a PrPSc immunoblot of tg66 mice (left panel) and tgRM mice (right panel) inoculated with either MV1 + 2C (cases 3 and 4) or MV2C (case 11) sCJD. Type 1 PrPSc accumulates in all of the recipient mice except for those inoculated with MV2C case 11 which accumulate Type 2 PrPSc. The numbers above the line indicate the case number while the numbers over the individual lanes represent the 3–4 brain samples that were analyzed for each CJD subtype inoculated. b PrPSc immunoblot of tg66 mice (left panel) and tgRM mice (right panel) inoculated with MV2K + 2C sCJD. Type 1 PrPSc accumulates in all of the recipient mice except for tg66 mice inoculated with case 9 (CC) which accumulate Type 2 PrPSc. The numbers above the line indicate the case number while the numbers over the individual lanes represent the 2–4 brain samples that were analyzed for each CJD subtype inoculated. Blots were developed using the mouse monoclonal anti-PrP antibody 3F4 conjugated to biotin. Lanes marked MV1 and MV2 are from human cases of sCJD and represent the controls for Type 1 and Type 2 PrPSc, respectively. Molecular mass markers are indicated on the right side of the figure

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